Description: We provide consultation and services for the following: 1. Multiparameter cell phenotyping up to 11-color antibody combinations. 2. Analysis applications such as cell activation, proliferation, cell cycle/DNA content, apoptosis, surface marker expressions, intracellular expressions, quantification of internal, regulatory and structural proteins and quantification of reporter genes (GFP, DsRed, mCherry, etc). 3. High-speed cell sorting of live and biohazardous samples, single-cell deposition into 96-well plates. Core instruments include: MoFlo XDP sorter, FACSAria II sorter, FACSCanto analyzers.
Progesterone is produced from cholesterol with pregnenolone as a metabolic intermediate . In the first step in the steroidogenic pathway , cholesterol is converted into pregnenolone, which serves as the precursor to the progestogens progesterone and 17α-hydroxyprogesterone. These progestogens, along with another steroid, 17α-hydroxypregnenolone , are the precursors of all other endogenous steroids, including the androgens, estrogens, glucocorticoids, mineralocorticoids, and neurosteroids. Thus, many tissues producing steroids, including the adrenal glands , testes , and ovaries , produce progestogens.